Protein Affinity Purification using Intein/Chitin Binding Protein Tags

Sarah F. Mitchell; Jon R. Lorsch

doi:10.1016/bs.mie.2014.11.002

Protein Affinity Purification using Intein/Chitin Binding Protein Tags

Sarah F. Mitchell, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Original language	English
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc.
Pages	111-125
Number of pages	15
DOIs	https://doi.org/10.1016/bs.mie.2014.11.002
State	Published - 2015
Externally published	Yes

Publication series

Name	Methods in Enzymology
Volume	559
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology

Keywords

CBD
Cell harvesting
Cell lysis
Chitin beads
IMPACT™-System
Intein/Chitin binding protein tags
Protein affinity purification

Access to Document

10.1016/bs.mie.2014.11.002

Cite this

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title = "Protein Affinity Purification using Intein/Chitin Binding Protein Tags",

keywords = "CBD, Cell harvesting, Cell lysis, Chitin beads, IMPACT{\texttrademark}-System, Intein/Chitin binding protein tags, Protein affinity purification",

author = "Mitchell, {Sarah F.} and Lorsch, {Jon R.}",

year = "2015",

doi = "10.1016/bs.mie.2014.11.002",

language = "English",

series = "Methods in Enzymology",

publisher = "Academic Press Inc.",

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TY - CHAP

T1 - Protein Affinity Purification using Intein/Chitin Binding Protein Tags

AU - Mitchell, Sarah F.

AU - Lorsch, Jon R.

PY - 2015

Y1 - 2015

KW - CBD

KW - Cell harvesting

KW - Cell lysis

KW - Chitin beads

KW - IMPACT™-System

KW - Intein/Chitin binding protein tags

KW - Protein affinity purification

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U2 - 10.1016/bs.mie.2014.11.002

DO - 10.1016/bs.mie.2014.11.002

M3 - Chapter

C2 - 26096506

AN - SCOPUS:84931570256

T3 - Methods in Enzymology

SP - 111

EP - 125

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -

Protein Affinity Purification using Intein/Chitin Binding Protein Tags

Publication series

ASJC Scopus Subject Areas

Keywords

Access to Document

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Cite this