Standard in vitro assays for protein-nucleic acid interactions - Gel shift assays for RNA and DNA binding

Sarah F. Mitchell, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Original languageEnglish
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages179-196
Number of pages18
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus Subject Areas

  • Biochemistry
  • Molecular Biology

Keywords

  • Coomassie blue staining
  • Electrophoretic mobility shift assay (EMSA)
  • Gel shift assays
  • In vitro assays
  • Poly(A)-binding protein (PABP)
  • Polyacrylamide gel preparation
  • Protein-nucleic acid interactions
  • RNA and DNA binding

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