The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier

Cory J Evans; Kayla L Bieser; Katherine S Acevedo-Vasquez; Emyli J Augustine; Skyler Bowen; Veronica A Casarez; Vanessa I Feliciano; Ashley Glazier; Haley R Guinan; Randy Hallman; Elizabeth Haugan; Lauren A Hehr; Shawna N Hunnicutt; Isabella Leifer; Meaghan Mauger; Morgan Mauger; Norma Y Melendez; Larry Milshteyn; Eric Moore; Sarah A Nguyen; Sierra C Phanphouvong; David M Pinal; Hailee M Pope; Mark-Brandon M Salinas; Matthew Shellin; Ivana Small; Neelufar C Yeoh; Alexandra M K Yokomizo; Jacob D Kagey

doi:10.17912/micropub.biology.000653

The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier

Cory J Evans, Kayla L Bieser, Katherine S Acevedo-Vasquez, Emyli J Augustine, Skyler Bowen, Veronica A Casarez, Vanessa I Feliciano, Ashley Glazier, Haley R Guinan, Randy Hallman, Elizabeth Haugan, Lauren A Hehr, Shawna N Hunnicutt, Isabella Leifer, Meaghan Mauger, Morgan Mauger, Norma Y Melendez, Larry Milshteyn, Eric Moore, Sarah A NguyenSierra C Phanphouvong, David M Pinal, Hailee M Pope, Mark-Brandon M Salinas, Matthew Shellin, Ivana Small, Neelufar C Yeoh, Alexandra M K Yokomizo, Jacob D Kagey

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.

Original language	English
Journal	microPublication biology
Volume	2022
DOIs	https://doi.org/10.17912/micropub.biology.000653
State	Published - 2022

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10.17912/micropub.biology.000653

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Evans, C. J., Bieser, K. L., Acevedo-Vasquez, K. S., Augustine, E. J., Bowen, S., Casarez, V. A., Feliciano, V. I., Glazier, A., Guinan, H. R., Hallman, R., Haugan, E., Hehr, L. A., Hunnicutt, S. N., Leifer, I., Mauger, M., Mauger, M., Melendez, N. Y., Milshteyn, L., Moore, E., ... Kagey, J. D. (2022). The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier. microPublication biology, 2022. https://doi.org/10.17912/micropub.biology.000653

Evans, CJ, Bieser, KL, Acevedo-Vasquez, KS, Augustine, EJ, Bowen, S, Casarez, VA, Feliciano, VI, Glazier, A, Guinan, HR, Hallman, R, Haugan, E, Hehr, LA, Hunnicutt, SN, Leifer, I, Mauger, M, Mauger, M, Melendez, NY, Milshteyn, L, Moore, E, Nguyen, SA, Phanphouvong, SC, Pinal, DM, Pope, HM, Salinas, M-BM, Shellin, M, Small, I, Yeoh, NC, Yokomizo, AMK & Kagey, JD 2022, 'The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier', microPublication biology, vol. 2022. https://doi.org/10.17912/micropub.biology.000653

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title = "The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier",

abstract = "The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.",

author = "Evans, {Cory J} and Bieser, {Kayla L} and Acevedo-Vasquez, {Katherine S} and Augustine, {Emyli J} and Skyler Bowen and Casarez, {Veronica A} and Feliciano, {Vanessa I} and Ashley Glazier and Guinan, {Haley R} and Randy Hallman and Elizabeth Haugan and Hehr, {Lauren A} and Hunnicutt, {Shawna N} and Isabella Leifer and Meaghan Mauger and Morgan Mauger and Melendez, {Norma Y} and Larry Milshteyn and Eric Moore and Nguyen, {Sarah A} and Phanphouvong, {Sierra C} and Pinal, {David M} and Pope, {Hailee M} and Salinas, {Mark-Brandon M} and Matthew Shellin and Ivana Small and Yeoh, {Neelufar C} and Yokomizo, {Alexandra M K} and Kagey, {Jacob D}",

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year = "2022",

doi = "10.17912/micropub.biology.000653",

language = "English",

volume = "2022",

journal = "microPublication biology",

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T1 - The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier

AU - Evans, Cory J

AU - Bieser, Kayla L

AU - Acevedo-Vasquez, Katherine S

AU - Augustine, Emyli J

AU - Bowen, Skyler

AU - Casarez, Veronica A

AU - Feliciano, Vanessa I

AU - Glazier, Ashley

AU - Guinan, Haley R

AU - Hallman, Randy

AU - Haugan, Elizabeth

AU - Hehr, Lauren A

AU - Hunnicutt, Shawna N

AU - Leifer, Isabella

AU - Mauger, Meaghan

AU - Mauger, Morgan

AU - Melendez, Norma Y

AU - Milshteyn, Larry

AU - Moore, Eric

AU - Nguyen, Sarah A

AU - Phanphouvong, Sierra C

AU - Pinal, David M

AU - Pope, Hailee M

AU - Salinas, Mark-Brandon M

AU - Shellin, Matthew

AU - Small, Ivana

AU - Yeoh, Neelufar C

AU - Yokomizo, Alexandra M K

AU - Kagey, Jacob D

PY - 2022

Y1 - 2022

N2 - The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.

AB - The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.

U2 - 10.17912/micropub.biology.000653

DO - 10.17912/micropub.biology.000653

M3 - Article

C2 - 36389120

SN - 2578-9430

VL - 2022

JO - microPublication biology

JF - microPublication biology

ER -